| N-Osteopontin

Osteopontin (OPN) is not only a secreted matricellular cytokine, but also a circulating pro-inflammatory cytokine, which can induce cell adhesion, migration, and activation of inflammatory pathways. Chronic increase of OPN expression is associated with an increased risk of major adverse cardiovascular events. During inflammatory cell infiltration, OPN can be cleaved by thrombin into two fragments at 168R/S169 site, one of which is the N-terminal fragment of OPN (N-OPN) which exposes a cryptic integrin binding motif, 162SVVYGLR168 (SLAYGLR in mice), and binds to α4β1 and α9β1 integrins, thereby exerting a strong pro-inflammatory effect.N-OPN plays a key role in multiple sclerosis by increasing the production of interleukin (IL)-17 and IL-6.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human N-Osteopontin spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human N-Osteopontin and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108